Santa Giustina di Venezia is a church building in the sestiere of Castello, Venice. It no longer functions as a church.
It was initially rebuilt in the second half of the 15th century by Augustinian nuns. The Convent was suppressed in 1896. The building garlanded with Istrian marble, was initially commissioned by the procurator of St. Mark, Giovanni Soranzo, who asked Baldassare Longhena to design and build the church in 1636-77.